Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Identification of G6PD Aures by an ARMS-PCR assay. a Schematic representation of the primer location and predicted size of PCR products for the G6PD Aures and internal control fragment. b Agarose gel electrophoresis represents band of G6PD Aures (226 bp) and internal control fragments (519 bp). Lane 1 represents the 100 bp DNA marker. Lanes 2–4 represent G6PD Aures-positive samples, and lanes 5–6, G6PD Aures-negative samples

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Control, Agarose Gel Electrophoresis, Marker

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Agarose gel electrophoresis representing 8 G6PD Asian genotypes. Lane 1 represents a 100 bp DNA marker. Lane 2 is a G6PD mutation standard marker. Lanes 3–7 are PCR products of unknown samples, lane 8 is the normal control, and lane 9 is the mutant positive control

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Agarose Gel Electrophoresis, Marker, Mutagenesis, Control, Positive Control

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Prevalence and frequency of G6PD gene mutations in the Lao Theung ethnic group

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques:

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: The histogram shows the correlation between G6PD activity (U/gHb) and G6PD mutations in the Lao Theung population. a G6PD activity (U/gHb) in each G6PD mutation group for males. Enzyme activity below 2.0 U/gHb indicates G6PD deficiency; enzyme activity of 2.0 to 4.67 U/gHb indicates G6PD intermediate. All hemizygous subjects presented G6PD deficiency. b G6PD activity for the female population. Homozygous subjects presented G6PD deficiency, while the heterozygous subjects have intermediate to normal G6PD enzyme activity

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Activity Assay, Mutagenesis

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Prevalence and molecular characteristics of G6PD deficiency in Lao PDR

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Mutagenesis

Journal: PLoS ONE

Article Title: Evaluation of the Diagnostic Accuracy of CareStart G6PD Deficiency Rapid Diagnostic Test (RDT) in a Malaria Endemic Area in Ghana, Africa

doi: 10.1371/journal.pone.0125796

Figure Lengend Snippet: An outline comparing some common factors of importance between the methods used for G6PD screening in the study.

Article Snippet: The CareStart G6PD deficiency RDT and Trinity Quantitative methods were controlled with two levels of commercially prepared G6PD quality control (QC) (normal level, Cat. No. PD 2618 and deficient level Cat. No. PD 2617 controls) samples from Randox.

Techniques:

Journal: PLoS ONE

Article Title: Evaluation of the Diagnostic Accuracy of CareStart G6PD Deficiency Rapid Diagnostic Test (RDT) in a Malaria Endemic Area in Ghana, Africa

doi: 10.1371/journal.pone.0125796

Figure Lengend Snippet: Performance of the CareStart G6PD deficiency RDT kit to other commercially available methods for screening G6PD deficiency .

Article Snippet: The CareStart G6PD deficiency RDT and Trinity Quantitative methods were controlled with two levels of commercially prepared G6PD quality control (QC) (normal level, Cat. No. PD 2618 and deficient level Cat. No. PD 2617 controls) samples from Randox.

Techniques:

Journal: PLoS ONE

Article Title: Evaluation of the Diagnostic Accuracy of CareStart G6PD Deficiency Rapid Diagnostic Test (RDT) in a Malaria Endemic Area in Ghana, Africa

doi: 10.1371/journal.pone.0125796

Figure Lengend Snippet: Diagnostic performance of CareStart G6PD RDT by gender compared to Trinity Biotech Quantitative and Qualitative methods for G6PD deficiency screening.

Article Snippet: The CareStart G6PD deficiency RDT and Trinity Quantitative methods were controlled with two levels of commercially prepared G6PD quality control (QC) (normal level, Cat. No. PD 2618 and deficient level Cat. No. PD 2617 controls) samples from Randox.

Techniques: Diagnostic Assay

Journal: PLoS ONE

Article Title: Evaluation of the Diagnostic Accuracy of CareStart G6PD Deficiency Rapid Diagnostic Test (RDT) in a Malaria Endemic Area in Ghana, Africa

doi: 10.1371/journal.pone.0125796

Figure Lengend Snippet: Area under the Receiver Operator Characteristics (ROC) curve for G6PD RDT performance considering malaria status of participants.

Article Snippet: The CareStart G6PD deficiency RDT and Trinity Quantitative methods were controlled with two levels of commercially prepared G6PD quality control (QC) (normal level, Cat. No. PD 2618 and deficient level Cat. No. PD 2617 controls) samples from Randox.

Techniques:

Journal: PLoS ONE

Article Title: Evaluation of the Diagnostic Accuracy of CareStart G6PD Deficiency Rapid Diagnostic Test (RDT) in a Malaria Endemic Area in Ghana, Africa

doi: 10.1371/journal.pone.0125796

Figure Lengend Snippet: Estimates of G6PD enzyme activity levels by malaria parasite status.

Article Snippet: The CareStart G6PD deficiency RDT and Trinity Quantitative methods were controlled with two levels of commercially prepared G6PD quality control (QC) (normal level, Cat. No. PD 2618 and deficient level Cat. No. PD 2617 controls) samples from Randox.

Techniques: Activity Assay